Cardio/ION Profile

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Category:

Turnaround Time: 21-24 Days
Test Type: Kit Based - Serum, plasma, urine

Overview:

The Cardio ION is a combination of nutritional tests that gives a nutritional assessment and identifies risk factors for cardiovascular disease (CVD). Specifically, this nutritional test measures levels of fatty acids, amino acids, vitamins, minerals, antioxidants, organic acids, and risk factors for CVD.

The Cardio ION nutritional test identifies modifiable risk factors for CVD, including:

  • Fatty acid imbalances which can lead to chronic inflammation. Current research finds levels of specific fatty acids to be strongly correlated to CVD.
  • High fibrinogen is associated with increased risk of clots and CVD
  • Homocysteine is an important risk factor for not only CVD, but stroke and dementia as well
  • Coenzyme Q10 (CoQ10) is essential for proper mitochondrial function and energy use. It also acts as an antioxidant. Cardiac cells have high mitochondrial demand. Statins can decrease CoQ10 levels.
  • Fasting insulin is an indicator of insulin resistance. Hyperinsulinaemia has been associated with a cluster of CVD risk factors.
  • Insufficient antioxidant status is associated with increased oxidation. Oxidative damage is believed to play a role in heart and vascular disease by promoting inflammation.

The Cardio ION nutritional test can guide treatment of many conditions and symptoms, including:

  • Chronic Fatigue Syndrome
  • Obesity
  • Immune Disorders
  • Mental/Emotional Disorders
  • Learning and Behavior Disorders
  • Multiple Chemical Sensitivities
  • Inflammatory Conditions

Why Order the Cardio ION nutritional test?

An evaluation of standard lipid markers does not provide optimal assessment of risk. The Cardio ION nutritional test goes beyond conventional serum lipid markers! This nutritional test identifies critical CVD risk factors, including:

  • Testosterone
  • RBC Magnesium
  • EPA and DHA levels
  • CoQ10
  • Insulin
  • Fibrinogen
  • C-Reactive Protein
  • Homocysteine
  • Lipoprotein(a)
  • AA/EPA Ratio
  • Vitamin D
  • Vitamin E (alpha tocopherol and gamma tocopherol)*

*Not available in New York

Performing Lab: Genova

Collection Details:

Patient Preparation:


• It is best to ship your specimen within 48 hours of collection. Please refer to the
enclosed shipping instructions before you collect to determine what days you can
ship your specimen.
• It is not necessary to discontinue nutritional supplements prior to this specimen
collection. Abnormalities that may be found will reveal special needs that have
not been met by recent dietary and supplemental intake.
• Decrease fluid intake to avoid excessive dilution of the urine.
» For adults, restrict intake to three 8 oz. glasses or less for 24 hours.
» Make sure that no more than 8 oz. of fluids are consumed after 8:00, the
evening prior to urine collection.
• Do not collect urine during menstruation.
• Vial contains preservative - Do Not Rinse.
Urine Collection
1. Write patient’s first and last name, date of birth, gender and date of collection on
the Test Requisition Form (located in the pouch on top of the Specimen Collection
Kit Box), as well as on all tube(s) and/or vial(s), using a permanent marker.
• IMPORTANT: To ensure accurate test results, you must provide the requested
information.
2. Empty bladder before going to bed at night. DO NOT collect this urine.
3. Collect urine (if any) during the night and first morning urine into a clean container.
4. Pipette urine, using a fresh disposable pipette, into the clear cap plastic vial to the
12 ml mark (DO NOT OVERFILL). Screw the cap on tightly.
5. Dispose of the remaining urine.
6. Freeze the clear cap plastic vial and the ice packet.

Collection Instructions:

1. Write patient’s first and last name, date of birth, gender and date of collection on
the Test Requisition Form (located in the pouch on top of the Specimen Collection
Kit Box), as well as on all tube(s) and/or vial(s), using a permanent marker.
• IMPORTANT: To ensure accurate test results, you must provide the requested
information.
2. Freeze the ice packets.
3. Light-blue top Na-citrate tube
• Draw the light-blue top Na-citrate tube completely.
• Invert the tube gently 10 times immediately after the draw.
• Centrifuge immediately for 15 minutes. The plasma must be free of hemolysis or
red blood cells.
• Remove the light-blue top Na-citrate tube after centrifuging;
DO NOT INVERT THE TUBE.
• Pipette plasma, using a fresh disposable pipette, 2.5 - 3 ml from the light-blue top
Na-citrate tube into the light-blue top clear transfer tube. Cap tightly.
• Freeze the light-blue top clear transfer tube
4. Red/gray top serum separator tubes and red top amber transfer tubes
• Draw the 4 red/gray top serum separator tubes .
• Place upright in a rack at room temperature for 20 to 30 minutes to clot blood.
• Centrifuge the red/gray top serum separator tubes for 15 minutes. The serum
must be free of hemolysis or red blood cells.
• Pipette all of the serum off of the 4 red/gray separator tubes into the 4 red top
amber transfer tubes. (DO NOT fill more than ¾ full to allow for freezing). Cap
each tube tightly.
• Freeze the red top amber transfer tubes.
5. Royal-blue top Na-EDTA tube, trace mineral free
• Draw the royal-blue top Na-EDTA tube completely.
• Invert the tube gently 15 times immediately after the blood draw.
• Leave the tube at room temperature. Do not centrifuge or transfer.
6. Lavender top EDTA tube and lavender top clear transfer tube
• Draw the lavender top EDTA tube completely.
• Invert the lavender top EDTA tube 10 times to mix the EDTA with the blood.
• Centrifuge immediately for 15 minutes. The plasma must be free of hemolysis or
red blood cells.
• Remove the lavender top EDTA tube after centrifuging;
DO NOT INVERT THE TUBE
• Pipette plasma, using a fresh disposable pipette, 2.5 - 3 ml to the lavender top
clear transfer tube
• Freeze the lavender top clear transfer tube
Specimen Preparation
1. Place all of the frozen transfer tubes, and the frozen urine collection into the slots
or the ends of the plastic shell tube tray (an exact fit is not necessary). Place the
absorbent pads over the tubes. Place the frozen ice packets at each end of the tubes
in the tray and one in the middle. Snap the tray closed. (Do not place the royal-blue
top Na-EDTA tube inside the tray).
2. Place the tray, along with the royal-blue top Na-EDTA tube, into the biohazard bag.
3. Staple payment to the bottom right-hand corner of the completed Test Requisition
Form and complete the Personal Health Assessment Form; Fold and Place them in
the side pocket of the biohazard bag.
4. Seal the biohazard bag, Place it into the specimen collection kit box, and close the
box.
Please read all instructions carefully before you begin.
Patient Preparation
• It is best to ship the specimen within 48 hours of collection. Please refer to the
enclosed shipping instructions before you collect to determine the days that the
specimen can be shippped.
• Please check to make sure the patient has fasted prior to drawing blood
Blood Collection
1. Write patient’s first and last name, date of birth, gender and date of collection on
the Test Requisition Form (located in the pouch on top of the Specimen Collection
Kit Box), as well as on all tube(s) and/or vial(s), using a permanent marker.
• IMPORTANT: To ensure accurate test results, you must provide the requested
information.
2. Freeze the ice packets.
3. Light-blue top Na-citrate tube
• Draw the light-blue top Na-citrate tube completely.
• Invert the tube gently 10 times immediately after the draw.
• Centrifuge immediately for 15 minutes. The plasma must be free of hemolysis or
red blood cells.
• Remove the light-blue top Na-citrate tube after centrifuging;
DO NOT INVERT THE TUBE.
• Pipette plasma, using a fresh disposable pipette, 2.5 - 3 ml from the light-blue top
Na-citrate tube into the light-blue top clear transfer tube. Cap tightly.
• Freeze the light-blue top clear transfer tube
4. Red/gray top serum separator tubes and red top amber transfer tubes
• Draw the 4 red/gray top serum separator tubes .
• Place upright in a rack at room temperature for 20 to 30 minutes to clot blood.
• Centrifuge the red/gray top serum separator tubes for 15 minutes. The serum
must be free of hemolysis or red blood cells.
• Pipette all of the serum off of the 4 red/gray separator tubes into the 4 red top
amber transfer tubes. (DO NOT fill more than ¾ full to allow for freezing). Cap
each tube tightly.
• Freeze the red top amber transfer tubes.
5. Royal-blue top Na-EDTA tube, trace mineral free
• Draw the royal-blue top Na-EDTA tube completely.
• Invert the tube gently 15 times immediately after the blood draw.
• Leave the tube at room temperature. Do not centrifuge or transfer.
6. Lavender top EDTA tube and lavender top clear transfer tube
• Draw the lavender top EDTA tube completely.
• Invert the lavender top EDTA tube 10 times to mix the EDTA with the blood.
• Centrifuge immediately for 15 minutes. The plasma must be free of hemolysis or
red blood cells.
• Remove the lavender top EDTA tube after centrifuging;
DO NOT INVERT THE TUBE
• Pipette plasma, using a fresh disposable pipette, 2.5 - 3 ml to the lavender top
clear transfer tube
• Freeze the lavender top clear transfer tube
Specimen Preparation
1. Place all of the frozen transfer tubes, and the frozen urine collection into the slots
or the ends of the plastic shell tube tray (an exact fit is not necessary). Place the
absorbent pads over the tubes. Place the frozen ice packets at each end of the tubes
in the tray and one in the middle. Snap the tray closed. (Do not place the royal-blue
top Na-EDTA tube inside the tray).
2. Place the tray, along with the royal-blue top Na-EDTA tube, into the biohazard bag.
3. Staple payment to the bottom right-hand corner of the completed Test Requisition
Form and complete the Personal Health Assessment Form; Fold and Place them in
the side pocket of the biohazard bag.
4. Seal the biohazard bag, Place it into the specimen collection kit box, and close the
box.