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The Bartonella henselae Polymerase Chain Reaction (PCR) test is an assay that detects B. henselae-specific DNA in whole blood and/or cerebral spinal fluid (CSF). The combination of the following three steps imparts a very high specificity and sensitivity to the test:
Principle: B. henselae ribosomal DNA (rDNA) fragments are hybrid-selected by probes, followed by PCR amplification of selected B. henselae rDNA. PCR products are confirmed by B. henselae-specific probes in a southern blot assay. The primers and probes used for the selection of B. henselae rDNA fragments are designed from published small subunit ribosomal RNA sequences.
Stage of Disease: Any Stage of Disease
Test Methodology: PCR
Collection Instructions:
Specimen: Urine – BD Gray Top/ 4mL x2
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