B. henselae PCR Urine (282)

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Test Type: Kit Based - Urine


The Bartonella henselae Polymerase Chain Reaction (PCR) test is an assay that detects B. henselae-specific DNA in whole blood and/or cerebral spinal fluid (CSF). The combination of the following three steps imparts a very high specificity and sensitivity to the test:

  1. Hybridization/selection
  2. Amplification of Bartonella-specific DNA
  3. Detection of Bartonella-specific amplified DNA fragments

Principle: B. henselae ribosomal DNA (rDNA) fragments are hybrid-selected by probes, followed by PCR amplification of selected B. henselae rDNA. PCR products are confirmed by B. henselae-specific probes in a southern blot assay. The primers and probes used for the selection of B. henselae rDNA fragments are designed from published small subunit ribosomal RNA sequences.


Stage of Disease: Any Stage of Disease

Test Methodology: PCR

Collection Details:

Collection Instructions:

Specimen:  Urine – BD Gray Top/ 4mL x2