Bordetella pertussis, Nasopharyngeal Culture

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Turnaround Time: 13 - 16 days
CPT Code:


Test Type: Nasopharyngeal swab, One swab


Isolate and identify B pertussis and B parapertussis; establish diagnosis of whooping cough.

Nucleic Acid Amplification (NAA/PCR) procedures provide morerapid results and have been increasingly used in the diagnosis of B pertussis infection. Studies have shown that the best yield is obtained when PCR and culture are used to diagnose this infection.

Collection Details:

Patient Preparation:

Patient must not be on antimicrobial therapy.

Collection Instructions:

The request form must state evaluate for Bordetella pertussis.

Flexible swab and special charcoal-containing.

Bordetella transport media (available from laboratory). Guide the flexible swab into the contour of the nares and into the nasopharynx. Pass the swab gently through the nose. Leave swab in place near septum and floor of nose for 15 to 30 seconds. Rotate and remove. Place into special transport media available from the laboratory.

If possible, preincubate at 37(degrees)C; then use refrigerated temperature (2 degrees Celsius to 8 degrees Celsius) for transport.