Folate is necessary for normal metabolism, DNA synthesis and red blood cell regeneration. Untreated deficiencies may lead to megaloblastic anemia. One of the most important folate-dependent reactions is the conversion of homocysteine to methionine.
Electrochemiluminescence Immunoassay (ECLIA)
Isolated folate deficiency is uncommon; it usually coexists with other nutrient deficiencies because of its strong association with poor diet, alcoholism, and, sometimes, malabsorptive disorders. Women with insufficient folate intakes are at increased risk of giving birth to infants with neural tube defects.
- High doses of biotin (> 5 mg/day) may interfere with assay results. Patient assumed to be refraining from biotin supplementation for at least 3 days prior to blood draw.