Gestational Glucose Tolerance Diagnostic Test (Three-hour, ACOG Recommendations)

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Turnaround Time: Within 1 day
CPT Code:

82951; 82952

Test Type: 1 mL serum or plasma each tube Serum or plasma
Stability Time:



Room temperature

14 days


14 days


14 days

Freeze/thaw cycles

Stable x3


Diagnose gestational diabetes.

In 2013,2 the American College of Obstetricians and Gynecologists (ACOG) recommended that all pregnant women be screened for gestational diabetes mellitus (GDM)—whether by patient history, clinical risk factors, or with a 50-gram, one-hour loading test at 24 to 28 weeks of gestation to determine blood glucose levels—and suggested relying on the result of the 100-gram, three-hour oral glucose tolerance test for diagnosis (often referred to as a "two-step" method).

The American Diabetes Association (ADA)1 released standards that vary from the ACOG recommendations. The ADA standards recommend a simplified "one-step" approach to the screen and diagnosis of gestational diabetes mellitus with a 75-gram, two-hour glucose tolerance test.

The LabCorp test according to the ADA recommendations is Gestational Glucose Tolerance Screening and Diagnostics Test (Two-hour, ADA Recommendations) [101000].

1. American Diabetes Association. Standards of Medical Care in Diabetes—2015: Summary of revisions. Diabetes Care. 2015 Jan; 38(Suppl 1):S4. PubMed 25537706

2. American College of Obstetricians and Gynecologists. Committee on Practice Bulletins—Obstetrics. Practice Bulletin N° 137: Gestational diabetes mellitus. Obstet Gynecol. 2013 Aug; 122(2 Pt 1):406-416. PubMed 23969827

3. LabCorp internal studies.

Collection Details:

Patient Preparation:

For three days prior to the test, patients should eat an unrestricted diet of 150 or more grams of carbohydrate and be permitted unrestricted physical activity. Patients should be instructed to eat and drink nothing except water for at least eight hours and not more than 14 hours before the test. Patients should also be advised to discontinue, whenever possible, all nonessential medication that can affect glucose metabolism at least three days before testing.

Collection Instructions:

Gel-barrier tubes (4) or gray-top (sodium fluoride/potassium oxalate plasma) tubes (4).

Draw a fasting blood sugar before administering 100-gram glucose, and draw blood at one, two, and three hours. The subject should remain seated and not smoke throughout the test. Submit 1 mL serum or plasma for fasting, one-hour, two-hour, and three-hour specimens. Separate serum or plasma from cells within 45 minutes of venipuncture. Gray-top tubes only, may be submitted without centrifugation. Label each tube with patient's name and collection time interval (ie, fasting, one-hour, two-hour, and three-hour).

Maintain specimen at room temperature.3