Glyphosate Exposure Analysis

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Turnaround Time: 7-10 business days
Test Type: Kit Based - Urine


What is Glyphosate?

Glyphosate is the main active ingredient for many broad-spectrum herbicides. The increased use of glyphosate in agriculture, recreational areas, and home gardens has increased 100-fold in the last 20 years. This has led to increased detection of glyphosate and its by-product aminomethylphosphoic acid (AMPA) in soil and water. There are over 750 different herbicides that contain glyphosate, which has made it difficult to avoid using products that contain it. The US EPA has raised the admissible glyphosate level in soy seeds from 2 ppm to 40 ppm. Human exposure is mainly through food. Glyphosate has been detected in human blood, urine, umbilical cord blood, and breast milk. The US National Nutrition Examination Survey found the herbicide in 80% of urine samples, however, the amount present varies widely. Although no safe limits have been set on glyphosate exposure, it would be advisable to limit exposure as much as possible. Glyphosate exhibits its herbicidal action through inhibition of the shikimate pathway enzyme EPSPS. Class I EPSPS are sensitive to the effects of glyphosate and are found in all plants and bacteria. However, glyphosate-resistant PSPS (Class II) appears to be more prevalent in opportunistic pathogens and may contribute to dysbiosis.


  • Cancer
  • Kills beneficial bacteria in the gut
  • Endocrine disrupter causing problems with hormone pathways

Collection Details:

Patient Preparation:

Collection Instructions

1. Make sure the test requisition form is COMPLETELY filled out EXCEPT for the very bottom of the page where it 1 says "RealTime Lab Use Only."

2. Collect urine into the conical cup provided (optional) approximately halfway full. A clean and/or new disposable cup can also be used.

3. Pour the urine into the plastic RTL tube. FILL ONLY HALF WAY FULL AND SECURELY SEAL THE ENCLOSED CAP. (You should hear a pop when it is fully secured).

4. Print on the specimen tube, in the designated areas: Date collected, patient's first AND last name, and patient's Date of Birth (DOB). SPECIMEN WILL BE REJECTED BY THE LAB IF THE TUBE IS BLANK!

5. Place tube in biohazard bag along absorbent pad and seal the top. Double check to ensure that the information on the tube matches the one on the test requisition form.

6. Fold and place paperwork in the outside sleeve of the biohazard bag. Note: this opening does not seal shut.

7. Place box inside pre-addressed FedEx/UPS/USPS sleeve and pull off the preventative stick tape at the top of the bag.

8. Make sure the bag is closed completely. 

All specimens should be sent immediately after collection (preferably within the first 48 hours after collection). If the specimen cannot be sent immediately, FREEZE the specimen and note the date it was frozen on the indicated part at the top of the test requisition. RTL validations allow for the specimen to be sent to the lab in a liquid state and received by the lab within 14 days of collection. The mailer provided by RTL is self-addressed and FedEx/UPS/USPS prepaid. There is no need to pay for shipping at the time of drop-off. The specimens may also be transported by overnight courier to the address below. RTL is not responsible for express delivery fees. RTL prepaid FedEx/UPS return labels may only be used within the continental United States.