Turnaround Time: 1 - 3 days

CPT Code:

86359, 86360, 86357

Test Type: Whole blood, Fill tube(s) to capacity.

Stability Time:

Temperature	Period
Room temperature	2 days
Refrigerated	Unstable
Frozen	Unstable
Freeze/thaw cycles	Unstable

Reference Range:

Reference intervals for adults have been established by the laboratory. See table.

Reference Intervals for Lymphocyte Immunophenotyping

Overview:

Monitor patients helper/suppressor T-cell status; assess other changes in T-cell surface markers that may indicate changes in immune status.

HIV-1 infection results in a decrease of CD4 T cells, an increase in CD8 T cells, a decrease in the CD4:CD8 ratio, and a progressive destruction of immune function. Enumeration of CD4 and CD8 T cells in HIV-1 seropositive patients may be used for prognostic purposes and to monitor disease progression and retroviral therapy. Natural killer (NK) cells are large granular lymphocytes that mediate MHC-unrestricted cytotoxicity against virus-infected and malignant cells and manufacture a number of cytokines following stimulation of the immune system.

Centers for Disease Control and Prevention. MMWR Recomm Rep. 1997 Jan 10;46(RR-2):1-29. PubMed 9011774

Giorgi J, Kesson A, Chou CC. Immunodeficiency and infectious diseases. In: Rose N, deMacario E, Fahey J, et al, eds. Manual of Clinical Laboratory Immunology. 4th ed. Washington, DC: ASM Press;1992:chap 25.

Lanier L, Jackson A. Monoclonal antibodies: Differentiation antigens expressed on leukocytes. In: Rose N, deMacario E, Fahey J, et al, eds. Manual of Clinical Laboratory Immunology. 4th ed. Washington, DC: ASM Press;1992:chap 23.

Stevens RA, Lempicki RA, Natarajan V, et al. General immunologic evaluation of patients with immunodeficiency virus infection. In: Detrick B, Hamilton RG, Folds JD, et al, eds. Manual of Molecular and Clinical Laboratory Immunology. 7th ed. Washington, DC: ASM Press;2006:chap 95.

Collection Details:

Collection Instructions:

Lavender-top (EDTA) tube and yellow-top (ACD-A or ACD-B) tube.

Invert tube 8 to 10 times immediately after collection. To preserve cellular viability, collect specimen so it will arrive in the laboratory within 48 hours of collection. Indicate date and time of venipuncture on the tube(s) and on the test request form.

Maintain specimen at room temperature. Do not freeze or refrigerate.

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CD	Age	95% Confidence Interval
		Percentage		Cells/mm³
		Minimum	Maximum	Minimum	Maximum
CD3⁺	Neonates	28	76	600	5000
	1 wk to 2 m	60	85	2300	7000
	2 to 5 m	48	75	2300	6500
	5 to 9 m	50	77	2400	6900
	9 to 15 m	54	76	1600	6700
	15 to 24 m	39	73	1400	8000
	2 to 5 y	43	76	900

Helper/Suppressor and Natural Killer Cell Profile

Collection Details:

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