Hemoglobin (Hb) Solubility With Reflex to Hemoglobin Fractionation, Blood*

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Turnaround Time: 1 - 3 days
CPT Code:


Test Type: 1 mL Whole blood
Stability Time:



Room temperature

14 days


14 days


14 days

Freeze/thaw cycles

Stable x3

Reference Range:



Qualitative determination of presence of hemoglobin S; detect sickling hemoglobins; evaluate hemolytic anemia, undiagnosed hereditary anemia with morphologic (sickle-like) abnormalities on peripheral blood smear.

Chehab FF, Kan YW. Detection of sickle cell anaemia mutation by colour DNA amplification. Lancet. 1990 Jan 6; 335(8680):15-17. PubMed 1967329

Miale JB. Laboratory Medicine: Hematology. 6th ed. St Louis, Mo: Mosby-Year Book Inc;1982:624-625.

Collection Details:

Patient Preparation:

No preliminary fluid or food restriction is required.

Collection Instructions:

Lavender-top (EDTA) tube; capillary puncture: three microhematocrit tubes. Blue-top (sodium citrate) tube, yellow-top (ACD) tube, or green-top (heparin) tube may also be submitted.

To avoid delays in turnaround time, please submit a separate lavender-top tube for each test requiring a lavender-top.

Room temperature.