Hemoglobinopathy Fractionation Cascade With Reflex to a-Thalassemia *

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CPT Code:

82728; 83020; 85027


Special Instructions:

This test is intended for adults age 20 and older. If MCV is <80 and ferritin is normal or high, testing will reflex to α-thalassemia DNA. If reflex test is performed, additional charges/CPT code(s) may apply.

This test may exhibit interference when sample is collected from a person who is consuming a supplement with a high dose of biotin (also termed as vitamin B7 or B8, vitamin H, or coenzyme R). It is recommended to ask all patients who may be indicated for this test about biotin supplementation. Patients should be cautioned to stop biotin consumption at least 72 hours prior to the collection of a sample.

Expected Turnaround Time:

2 - 5 days

Collection Details:

Collection Instructions:

Specimen Requirements


Whole blood and serum


Three lavender-top (EDTA whole blood) tubes filled to capacity and 0.8 mL serum

Minimum Volume

Two lavender-top (EDTA whole blood) tubes filled to capacity and 0.3 mL serum. (Note: This volume does not allow for repeat testing.)


Lavender-top (EDTA) tubes and red-top tube or gel-barrier tube

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