Category:

Turnaround Time: 1 - 3 days
CPT Code:

87350

Test Type: 1.5 mL Serum or Plasma
Stability Time:

Temperature

Period

Room Temperature

7 days (stability provided by manufacturer or literature reference)

Refrigerated

7 days (stability provided by manufacturer or literature reference)

Frozen

1 year (stability provided by manufacturer or literature reference)

Freeze/thaw cycles

Stable x3 (stability provided by manufacturer or literature reference)

Overview:

The HBe antigen assay, in conjunction with other serologicaland clinical information, is intended only for the determination of chronic infection with hepatitis B virus.

Assay performance characteristics have not been established for (1) children younger than 17 years of age, (2) pregnant women, or (3) populations of immunocompromised or immunosuppressed patients.

This assay has not been FDA-approved for the diagnosis of individuals with acute hepatitis B infection.

HBeAg appears in acute B hepatitis with or shortly after HBsAg, when the patient is most infectious. HBeAg is found only in HBsAg-positive sera. During the HBeAg-positive state, usually three to six weeks, hepatitis B patients are at increased risk of transmitting the virus to their contacts, including babies born during this period. Exposure to serum or body fluid positive for HBeAg and HBsAg is associated with three to five times greater risk of infectivity than when HBsAg positivity occurs alone. Persistence of HBeAg is associated with chronic liver disease.

Edwards MS. Hepatitis B serology−help in interpretation. Pediatr Clin North Am. 1988 Jun; 35(3):503-515 (review). PubMed 3287312

Lee HS, Vyas GN. Diagnosis of viral hepatitis. Clin Lab Med. 1987 Dec; 7(4):741-757 (review). PubMed 3319367

Mushahwar IK, Dienstag JL, Polesky HF, McGrath LC, Decker RH, Overby LR. Interpretation of various serological profiles of hepatitis B virus infection. Am J Clin Pathol. 1981 Dec; 76(6):773-777. PubMed 7315794

Mushahwar IK, McGrath LC, Drnec J, Overby LR. Radioimmunoassay for detection of hepatitis B e antigen and its antibody. Results of clinical evaluation. Am J Clin Pathol. 1981 Nov; 76(5):692-697. PubMed 6170222

Okada K, Kamiyama I, Inomata M, Imai M, Miyakawa Y. e antigen and anti-e in the serum of asymptomatic carrier mothers as indicators of positive and negative transmission of hepatitis B virus to their infants. N Engl J Med. 1976 Apr 1; 294(14):746-749. PubMed 943694

Collection Details:

Collection Instructions:

This test may exhibit interference when sample is collected from a person who is consuming a supplement with a high dose of biotin (also termed as vitamin B7 or B8, vitamin H, or coenzyme R). It is recommended to ask all patients who may be indicated for this test about biotin supplementation. Patients should be cautioned to stop biotin consumption at least 72 hours prior to the collection of a sample.

Gel-barrier tube OR lavender-top (EDTA) tube.

If tube other than a gel-barrier tube is used, transfer separated serum or plasma to a plastic transport tube.

Refrigerate.