Hepatitis C Virus (HCV), QT, RNA PCR (Graphical) w/Reflex to HCV NS5B Drug Resistance Assay*

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Turnaround Time: 11-17 Days
CPT Code:

87522. If reflex testing is performed, concomitant CPT codes/charges will apply.

Test Type: 4 mL Plasma (preferred) or serum, frozen


Quantification of HCV with reflex to HCV NS5B drug resistance assay (at additional charge) if ≥1000 IU/mL.

The HCV real-time PCR assay has a quantitative range of 15 IU/mL to 100,000,000 IU/mL. This assay requires an HCV genotype of either 1a or 1b.

Kleiber J, Walter T, Haberhausen G, Tsang S, Babiel R, Rosenstraus M. Performance characteristics of a quantitative, homogeneous TaqMan RT-PCR test for HCV RNA. J Mol Diagn. 2000 Aug; 2(3):158-166. PubMed 11229521

Collection Details:

Collection Instructions:

Please indicate the patient's HCV genotype (1a or 1b) on the test request form. Submit the patient's Social Security number (or unique identifier) to order serial monitoring.

Ship in plasma preparation tube (PPT™) or screw-capped polyproplylene frozen transport tubes.

Collect specimen in two PPT™ tubes, gel-barrier tubes, or lavender-top (EDTA) tubes. Do not use green-top (heparin) tubes. Centrifuge specimen within six hours of collection, remove serum/plasma, and transfer specimen to polypropylene screw-capped tubes, and freeze. Specimens drawn into PPT™ tubes need to be centrifuged within six hours and frozen. Ship frozen. To avoid delays in turnaround time when requesting multiple tests on frozen samples, please submit separate frozen specimens for each test requested.