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The Anaplasma phagocytophilum (HGA) IFA is designed to detect human IgG and IgM antibodies to HGA antigens in human serum. For diagnostic purposes, HGA IFA test results should be used in conjunction with other information available to the diagnosing physician.
The Anaplasma IFA assay is a two-stage sandwich assay, based upon an antigen-antibody complex formation in the following steps:
Principle: Titers rise during the first two-to-four weeks of illness and decline over the next six-to-12 months. In patients with previously high titers, the presence of only IgG titer of less than 160 may indicate a resolving infection. If the IFA result is negative, but the clinical symptoms of HGA infection are present, PCR testing is suggested. A. phagocytophilum is carried by the same ticks that have also been known to cause Babesiosis, Bartonellosis and Lyme disease. Patients with positive titers should also be tested for other tick-borne diseases.
Stage of Disease: Any Stage of Disease
Test Methodology: IFA