Histoplasma Galactomannan Antigen EIA, Urine

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Turnaround Time: 2 - 5 days
CPT Code:


Test Type: 5 mL Urine (unpreserved)


Diagnosis of histoplasmosis based upon detection of antigen.

A negative result does not preclude a diagnosis of histoplasmosis. This assay has been documented to cross-react with Blastomyces dermatitidis, Coccidioides immitis, Paracoccidioides brasiliensis, and Penicillium marneffei. Positive tests should be confirmed in areas or patient groups where these organisms are endemic or a risk. The following substances may interfere with the assay results: foods which produce color in urine; vaginal cream; caffeine; ascorbic acid, itraconazole; amphotericin B; acetaminophen; and acetylsalicylic acid.

This test was developed, and its performance characteristics determined, by LabCorp. It has not been cleared or approved by the US Food and Drug Administration (FDA).

Antigen detection may be useful in acute disease, especially in individuals also infected with HIV, who frequently have the disseminated form of histoplasmosis without detectable antibodies to the fungus. In chronic disease, antigen may not be detected due to the low fungal burden. The highest sensitivity for rapidly and accurately diagnosing histoplasmosis in patients with acute pulmonary or disseminated infection can be obtained by testing both urine and serum.

ALPHA Histoplasma EIA Test Kit for the Detection of Histoplasma Antigen- REF HAG102, Package Insert, Immuno- Mycologics, Inc., 2700 Technology Place, Norman, OK 73071, Revised 07/18/2011.

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Wheat LJ, Freifeld AG, Kleiman MB, et al. Clinical practice guidelines for the management of patients with histoplasmosis: 2007 update by the Infectious Diseases Society of America. Clin Infect Dis. 2007 Oct 1;45(7):807-825. PubMed 17806045

Wheat LJ, Kohler RB, Tewari RP. Diagnosis of disseminated histoplasmosis by detection of Histoplasma capsulatum antigen in serum and urine specimens. N Engl J Med. 1986 Jan 9;314(2):83-88. PubMed 3941695

Zhang X, Gibson B Jr, Daly TM. Evaluation of commercially available reagents for diagnosis of histoplasmosis infection in immunocompromised patients. J Clin Microbiol 2013 Dec;51(12):4095-4101. PubMed 24088857

Collection Details:

Collection Instructions:

Sterile cup or sterile screw-capped vial.

Collect specimen aseptically.

Refrigerate. Store specimens at 2°C to 8°C or frozen at -16°C to 24°C for up to 14 days.