Single Lab Test
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Washington, CT 06793
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Washington, CT 06793
Single Lab Test
The polymerase chain reaction (PCR)-based diagnostic assays are highly specific to Ehrlichia chaffeensis (HME) DNAs. The combination of the following three steps imparts a very high specificity and sensitivity to the test:
Principle: The Ehrlichia chaffeensis (HME) PCR test detects E. chaffeensis ribosomal DNA (rDNA) fragments in patient samples. The rDNA fragments are hybrid-selected by probes, followed by PCR amplification of the selected fragments using specific primers. PCR products are then confirmed with E. chaffeensis-specific probes by southern blot assay.
Stage of Disease: Any Stage of Disease
Test Methodology: PCR