HME (Ehrlichia chaffeensis) PCR Urine (780)

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Test Type: Kit Based - Urine


The polymerase chain reaction (PCR)-based diagnostic assays are highly specific to Ehrlichia chaffeensis (HME) DNAs. The combination of the following three steps imparts a very high specificity and sensitivity to the test:

  1. Hybridization/selection
  2. Amplification of Ehrlichia-specific DNA
  3. Detection of Ehrlichia-specific amplified DNA fragments

Principle: The Ehrlichia chaffeensis (HME) PCR test detects E. chaffeensis ribosomal DNA (rDNA) fragments in patient samples. The rDNA fragments are hybrid-selected by probes, followed by PCR amplification of the selected fragments using specific primers. PCR products are then confirmed with E. chaffeensis-specific probes by southern blot assay.


Stage of Disease: Any Stage of Disease

Test Methodology: PCR

Collection Details:

Collection Instructions:

Specimen:  Urine – BD Gray Top/ 4mL x2