Human Immunodeficiency Virus 1 & 2 (HIV-1/HIV-2), Qualitative, RNA

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Turnaround Time: 2-5 days
CPT Code:


Test Type: Plasma or Serum 1mL


This test is intended for use as an aid in the diagnosis of HIV-1 or HIV-2 infection, including acute or primary infection. Presence of HIV-1 or HIV-2 RNA in the plasma of patients without antibodies to HIV-1 or HIV-2 is indicative of acute or primary HIV-1 or HIV-2 infection. This assay may also be used as an additional test, when it is reactive, to confirm HIV-1 infection in an individual whose specimen is repeatedly reactive for HIV-1 and/or HIV-2 antibodies. This assay is not intended for use in donor testing.

Methodology: RT-PCR

Collection Details:

Collection Instructions:

Specimen: Plasma or serum

Volume: 1 mL

Minimum Volume: 650 µL

Container: Lavender-top (EDTA) tube, BD SST tube, BD Vacutainer PPT plasma preparation tube

Collection: Collect specimen in lavender-top (EDTA), gel-barrier (SST tube), or a PPT plasma preparation tube. Whole blood specimens stored at room temperature should be centrifuged within 24 hours of collection. Transfer serum or plasma to a polypropylene screw-cap tube for shipment.

Storage Instructions: Frozen. Sample may be stored for 24 hours at room temperature. It is recommended that serum or plasma be refrigerated or frozen for shipment.

Room temperature: 24 hours

Refrigerated: 7 days

Frozen: 6 weeks

Freeze/thaw cycles: Stable x3

Causes for Rejection: Specimen grossly hemolyzed or received outside of specimen and/or storage requirements