Human Immunodeficiency Virus 1 (HIV-1), Quantitative, RNA

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Category:

Turnaround Time: 3 - 7 days
CPT Code: 87536
Test Type: 3.5 mL Plasma, frozen
Stability Time:

Freeze; stable for long-term storage. Stable at room temperature for six hours or one day after centrifugation. Stable refrigerated for 24 hours or five days after centrifugation.

Overview:

Detect and quantitate HIV-1 in plasma.

Assay range: 40-10,000,000 copies/mL.

Huang S, Salituro J, Tang N, et al. Thermodynamically modulated partially double-stranded linear DNA probe design for homogeneous real-time PCR. Nucleic Acids Res. 2007; 35(16):e101. PubMed 17693434

Tang N, Huang S, Salituro J, et al. A RealTime HIV-1 viral load assay for automated quantitation of HIV-1 RNA in genetically diverse group M subtypes A-H, group O and group N samples. J Virol Methods. 2007; 146(1-2):236-245. PubMed 17707519

Collection Details:

Collection Instructions:

Ship in a screw-cap polypropylene frozen transport tube or centrifuged PPT(TM) tubes.

Collect specimen in two lavender-top (EDTA), PPT™, or yellow-top (ACD-A) tubes. Centrifuge specimen within six hours of collection when freshly drawn at 15°C to 30°C. If drawn in EDTA or ACD, the plasma must be transferred to a screw-cap polypropylene transport tube and frozen. If refrigerated, specimen may be held for 24 hours prior to centrifugation. Plasma specimens may be stored at -20°C for 60 days. If longer storage is required, plasma specimens must be stored at -70°C or lower. Ship frozen on dry ice. To avoid delays in turnaround time when requesting multiple tests on frozen samples, please submit separate frozen specimens for each test requested.

Freeze; stable for long-term storage. Stable at room temperature for six hours or one day after centrifugation. Stable refrigerated for 24 hours or five days after centrifugation.