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Lyme Dot-Blot Assay (LDA) is a qualitative immunoassay for the direct detection of Borrelia burgdorferi-specific antigens in a patient urine sample using anti-B. burgdorferi antibodies.
Purpose: The antigens present in the urine sample are immobilized onto a membrane in a Dot-blot format and incubated with anti-B. burgdorferi-specific antibodies. After washing the bound anti-B. burgdorferi-specific antibodies, they are reacted with anti-rabbit IgG, which is visualized by an enzyme/substrate reaction.
Stage of Disease: Any Stage of Disease
Test Methodology: LDA