Myeloperoxidase (MPO)

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Category:

Turnaround Time: 2 - 4 days
CPT Code:

83876

Test Type: 0.5 mL Plasma
Stability Time:

Temperature

Period

Refrigerated

5 days (stability determined by manufacturer or literature reference)

Frozen

6 months (stability determined by manufacturer or literature reference)
Reference Range:

• Low CVD Risk: <470 pmol/L

• Moderate Risk: 470–539 pmol/L

• High Risk: >539 pmol/L

Overview:

Elevated levels of plasma MPO are a sensitive indicator of inflammatory disorders.

Erratic results can be observed if plasma is not centrifuged and separated from red cells immediately.

Results of this test are labeled for "research purposes only." The result should not be used for treatment or for diagnostic purposes without confirmation of the diagnosis by another medically established diagnostic product or procedure. The performance characteristics were determined by LabCorp.

MPO plays an important role in the innate host-defense mechanism of human and animals.

Myeloperoxidate (MPO) is a hemoprotein present in leukocytes and catalyzes the hydrogen peroxide mediated peroxidation of halide ions to produce strong reactive oxidant species such as hypochlorous acid that are of potent antimicrobial activities against a broad range of invading parasites and pathogens. However, MPO-derived reactive oxidants also promote host tissue injury through lipid and protein peroxidations that lead to systemic inflammation.

Nilsson L, Brunnkvist S, Nilsson U, et al. Activation of inflammatory systems during cardiopulmonary bypass. Scand J Thorac Cardiovasc Surg. 1988;22(1):51-53. PubMed 2838898

Heinecke JW. Mechanisms of oxidative damage by myeloperoxidase in atherosclerosis and other inflammatory disorders. J Lab Clin Med. 1999 Apr;133(4):321-325. PubMed 10218761

Podil'chak MD, Terletskaia LM. Clinical value of determining myeloperoxidase and alkaline phosphatase activity of the leukocytes in patients with suppurative-inflammatory processes. Klin Khir. 1988;(1):59-60. PubMed 2835543

Re G, Azzimondi G, Lanzarini C, Bassein L, Vaona I, Guarnieri C. Plasma lipoperoxidative markers in ischaemic stroke suggest brain embolism. Eur J Emerg Med. 1997 Mar;4(1):5-9. PubMed 9152688

Biasucci LM, D'Onofrio G, Liuzzo G, et al. Intracellular neutrophil myeloperoxidase is reduced in unstable angina and acute myocardial infarction, but its reduction is not related to ischemia. J Am Coll Cardiol. 1996 Mar 1;27(3):611-616. PubMed 8606272

Shih J, Datwyler SA, Hsu SC, et al. Effect of collection tube type and preanalytical handling on myeloperoxidase concentrations. Clin Chem. 2008 Jun;54(6):1076-1079. PubMed 18509013

Collection Details:

Collection Instructions:

Lavender-top (EDTA) tube or green-top (heparin) tube.

Collect whole blood using venipuncture techniques. Gently mix the blood with the anticoagulant by inverting sample tube several times (do not shake).

Place freshly collected blood samples on ice or in a refrigerator 2°C to 8°C immediately, and store them at 2°C to 8°C until separation. Plasma should be physically separated from cells within two hours of collection by centrifugation at 2°C to 8°C. If a refrigerated centrifuge is not available, chill the centrifuge carrier in the refrigerator before use until cold.

Special precaution needs to be taken to ensure transfer of the plasma layer to a polypropylene (not glass) tube while avoiding carryover of any red blood cells or buffy coat white cells. The plasma samples thus prepared may be refrigerated at 2°C to 8°C for five days.

If plasma samples are prepared by gel based vacuum tubes, the plasma samples need to be transferred to separated tubes from the top of the gels immediately after the centrifugation.

Increased levels of MPO may be observed if the plasma samples are left on the top of the gels for more than eight hours before transferring to separate tubes.

Refrigerate.