Protein Electrophoresis With Interpretation, 24-Hour Urine

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Category:

Turnaround Time: 2 - 5 days
CPT Code:

84166, 84156

Test Type: 20 mL aliquot, Urine (24-hour)
Stability Time:

Temperature

Period

Refrigerated

3 days (stability provided by manufacturer or literature reference)

Frozen

1 month (stability provided by manufacturer or literature reference)

Reference Range:

Normal pattern. See table.

24-Hour Urine Protein Electrophoresis Interpretation

Pattern Description

Electrophoretic Zones

Total Protein

(mg/d)

Albumin

α1

α2

β

γ

Normal

<150

+/–

   

+/–

+/–

Selective glomerular proteinuria

           

mild

<1500

++

+

 

+

 

severe

>1500

+++

++

 

++

 

Nonselective glomerular proteinuria*

>3000

+++

++

++

++

++

Tubular proteinuria

<1500

Trace to +

Trace

++

++

Trace of light chain

Mixed glomerular and tubular proteinuria

 

+++

+

++

+

+

Albuminuria

>150

++

       

Overflow proteinuria from acute phase response

 

Trace or present

++

Overview:

Work up myeloma, macroglobulinemia of Waldenström, lymphoma, amyloidosis; differentiate between normal renal function, glomerular proteinuria, and tubular proteinuria. Increased glomerular permeability leads to higher concentrations of large proteins in the glomerular filtrate. Diminished tubular reabsorptive capacity results in a marked increase in urinary excretion of low molecular weight problems.

Collection Details:

Collection Instructions:

12-mL plastic transport tube (LabCorp N° 23597) without preservative.

Mix well.

Refrigerate at 2°C to 8°C.

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