Turnaround Time: 1 - 3 days

CPT Code:

86355; 86359; 86360

Test Type: Fill tube(s) to capacity. Whole blood

Stability Time:

Temperature	Period
Room temperature	2 days
Refrigerated	Unstable
Frozen	Unstable
Freeze/thaw cycles	Unstable

Reference Range:

Reference intervals for adults have been established by the laboratory. See table.

Reference Intervals for Lymphocyte Immunophenotyping

Overview:

Enumeration of lymphocyte subsets for classification and diagnosis of primary immunodeficiency diseases and for evaluating and monitoring AIDS patients.

Primary immunodeficiencies result from congenital defects of the immune system. Acquired immunodeficiency syndrome (AIDS) results from infection with the HIV-1 retrovirus. The purpose of flow cytometric enumeration is to determine whether select lymphocyte subsets are reduced or absent in order to support the diagnosis of numeric cellular immunodeficiency rather than immunodeficiency due to cellular dysfunction.

HIV-1 infection results in a decrease of CD4 T cells, an increase of CD8 T cells, a decrease in the CD4:CD8 ratio, and a progressive destruction of immune function. In HIV-1 seropositive patients, enumeration of CD4 T cells may be used for prognostic purposes and to monitor disease progression and antiretroviral therapy.

Centers for Disease Control and Prevention (CDC). 1997 revised guidelines for performing CD4+ T-cell determinations in persons infected with human immunodeficiency virus (HIV). MMWR. 1997 Jan 10; 46:RR-2.

Giorgi J, Kesson A, Chou CC. Immunodeficiency and infectious diseases. In: Rose N, deMacario E, Fahey J, et al, eds. Manual of Clinical Laboratory Immunology. 4th ed. Washington, DC: ASM Press;1992:chap 25.

Stevens RA, Lempicki RA, Natarajan V, et al. General immunologic evaluation of patients with immunodeficiency virus infection. In: Detrick B, Hamilton RG, Folds JD, et al, eds. Manual of Molecular and Clinical Laboratory Immunology. 7th ed. Washington, DC: ASM Press;2006:chap 95.

Collection Details:

Collection Instructions:

Lavender-top (EDTA) tube and yellow-top (ACD-A) or (ACD-B) tube.

Invert tube 8 to 10 times immediately after collection. To preserve cellular viability, collect specimen so it will arrive in the laboratory within 48 hours of collection. Indicate date and time of venipuncture on the tube(s) and on the test request form.

Maintain specimen at room temperature.

Discover the Evexia advantage Start ordering today

Take advantage of all the time and money-saving features that Evexia Diagnostics has to offer. Simply click the button below to get started.

Register Now

Contact Us

CD	Age	95% Confidence Interval
		Percentage		Cells/mm³
		Minimum	Maximum	Minimum	Maximum
CD3⁺	Neonates	28	76	600	5000
	1 wk to 2 m	60	85	2300	7000
	2 to 5 m	48	75	2300	6500
	5 to 9 m	50	77	2400	6900
	9 to 15 m	54	76	1600	6700
	15 to 24 m	39	73	1400	8000
	2 to 5 y	43	76	900	4500
	5 to 10 y	55	78	700	4200
	10 to 16 y	52	78	800	3500
	Adults	58	86	622

T- and B-Lymphocyte Differential Profile

Collection Details:

Discover the Evexia advantage Start ordering today