West Nile Virus (WNV), UltraQual® RT-PCR

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Category:

Turnaround Time: 10 - 14 days
CPT Code:

87798

Test Type: 3.5 mL Plasma, serum, or cerebrospinal fluid (CSF)

Overview:

Detect West Nile virus (WNV) RNA.

• Mean detection: 7.43 copies/mL

• 95% detection cutoff: 28.16 copies/mL

The direct detection of WNV is a valuable tool in determining whether a patient is infected with West Nile virus. It is also useful in determining whether blood and blood products are free of detectable WNV prior to distribution. PCR is able to detect WNV RNA directly and does not rely on antibody markers of WNV infection, which are produced after RNA is detectable in newly infected individuals.

Chomczynski P, Sacchi N. Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal Biochem. 1987 Apr; 162(1):156-159. PubMed 2440339

Kawasaki ES. Amplification of RNA. In Innis MA, Gelfand DH, Sninsky JJ, White TJ, eds. PCR Protocols: A Guide to Methods and Applications. San Diego, Calif: Academic Press;1990:21-28.

Collection Details:

Collection Instructions:

Note: Not available to NY clients.

Frozen transport tube or plasma preparation tube (PPT™).

Collect blood in gel-barrier tube, yellow-top (ACD) tube, lavender-top (EDTA) tube, or plasma preparation tube (PPT™). Centrifuge blood at 800−1600xg at room temperature within one hour of draw to separate serum or plasma from cells. Transfer serum or plasma to a plastic screw-capped transport tube and freeze. Specimens drawn into PPT™ need to be centrifuged in a swinging bucket rotor centrifuge within one hour and frozen. Ship frozen. To avoid delays in turnaround time when requesting multiple tests on frozen samples, please submit separate frozen specimens for each test requested.

Freeze.