Bordetella pertussis and Bordetella parapertussis, Real-time DNA PCR

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Turnaround Time: 2 -4 days
CPT Code:


Test Type: One NP/throat swab or 0.5 mL nasal aspirate/wash
Stability Time:



Room temperature

5 days


7 days


This assay is intended to be used as an aid to the diagnosis of Bordetella pertussis (whooping cough) and Bordetella parapertussis (whooping cough-like syndrome).

The target for the B pertussis PCR reaction, a region of IS481, is also found in Bordetella holmesii. A false-positive result for B pertussis DNA may occur if B holmesii is present in the sample tested.

Collection Details:

Collection Instructions:

• Option 1 (preferred): Flocked NP swab submitted dry in plastic transport sleeve (PeopleSoft N° 93307)

• Option 2 (alternate): NP swab submitted in charcoal-containing transport media

• Option 3 (alternate): NP/throat swab submitted in universal transport media (UTM)

• Option 4 (alternate): NP wash/aspirate submitted in sterile container

Maintain specimen at room temperature or refrigerate. Do not freeze charcoal transport swabs or dry swabs. Swabs in UTM and NP aspirates/washes may be frozen for seven days.