N-Terminal Pro-Brain Natriuretic Peptide (NT-proBNP) (1101)

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CPT Code:


Test Type: Serum Kit Based
Stability Time:


Refrigerated: 6 days

Reference Range:

Age: ≤75 yrs

  • Optimal: <125 pg/mL
  • Borderline: 125–450 pg/mL
  • Increased Risk: >450 pg/mL

Age: >75 yrs

  • Optimal: <450 pg/mL
  • Borderline: 450 pg/mL
  • Increased Risk: >450 pg/mL


Brain natriuretic peptide (BNP) is a neurohormone that affects body fluid homeostasis via diuresis and natriuresis. Elevated levels are seen in patients with congestive heart failure and a decreased ejection fraction.1 NT-proBNP is the precursor molecule for BNP.

Elevated levels Identify subjects with left ventricular dysfunction.1

  • Every incremental increase in values above normal is associated with an incremental increase in CV risk.
  • Levels >450 pg/mL in subjects with stable CAD are associated with a 2- to 6-fold increased risk for cardiac morbidity and/or mortality.
  • Normal levels exclude cardiac dysfunction.
  • High doses of biotin (> 5 mg/day) may interfere with assay results. The patient is assumed to be refraining from biotin supplementation for at least 3 days prior to the blood draw.


  1. Savarese G, Trimarco B, Dellegrottaglie S, et al. Natriuretic peptide-guided therapy in chronic heart failure: a meta-analysis of 2,686 patients in 12 randomized trials. PLoS One. 2013;8(3):e58287.

Methodology - Immunoassay

Collection Details:

Collection Instructions:

Preferred Specimen

1.0 mL serum (0.5 mL minimum) collected in serum separator tube (SST/Tiger Top)

Alternate Specimen

1.0 mL EDTA plasma (0.5 mL minimum) collected in plasma separator tube (Pearl Top).  Separate plasma as soon as possible after collection.

Transport Temperature

Refrigerated (ship on frozen cold packs)