Protein Electrophoresis, Serum and Quantitative Free ? and ? Light Chains (FLC) Plus Ratio, Serum

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Category:

Turnaround Time: 2 - 5 days
CPT Code:

84165, 84155, 83883(2)

Test Type: 2.5 mL Serum
Stability Time:

PE: Stable when covered at room temperature 25°C for four days; stable refrigerated at 2°C to 8°C for two weeks; stable frozen at -20°C for six months.

FLC: Refrigerate; samples are stable for 21 days at 2°C to 8°C. For longer storage, freeze at -20°C.

Reference Range:

Protein, Total, Serum g/dL 6.0 − 8.5
Albumin g/dL 2.9 − 4.4
Alpha−1−Globulin g/dL 0.0 − 0.4
Alpha−2−Globulin g/dL 0.4 − 1.0
Beta Globulin g/dL 0.7 − 1.3
Gamma Globulin g/dL 0.4 − 1.8
M−Spike g/dL Not Observed
Globulin, Total g/dL 2.2 − 3.9
A/G Ratio 0.7 − 1.7
Free Kappa Lt Chains,S mg/L 3.30 − 19.40
Free Lambda Lt Chains,S mg/L 5.71 − 26.30
Kappa/Lambda Ratio,S 0.26 − 1.65

Overview:

Detect and identify monoclonal immunoglobulin gammopathies.

Elevated serum levels of monoclonal FLC are associated with malignant plasma cell proliferation (eg, multiple myeloma), primary amyloidosis, and light chain deposition disease. Raised serum levels of polyclonal FLC may be associated with autoimmune diseases such as systemic lupus erythematosus.

Collection Details:

Collection Instructions:

Red-top tube or gel-barrier tube.

Sample should be allowed to clot and the serum separated as soon as possible to prevent hemolysis.

PE: Stable when covered at room temperature 25°C for four days; stable refrigerated at 2°C to 8°C for two weeks; stable frozen at -20°C for six months.

FLC: Refrigerate; samples are stable for 21 days at 2°C to 8°C. For longer storage, freeze at -20°C.