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Protein, Total, Serum g/dL 6.0 − 8.5
Albumin g/dL 2.9 − 4.4
Alpha−1−Globulin g/dL 0.0 − 0.4
Alpha−2−Globulin g/dL 0.4 − 1.0
Beta Globulin g/dL 0.7 − 1.3
Gamma Globulin g/dL 0.4 − 1.8
M−Spike g/dL Not observed
Globulin, Total g/dL 2.2 − 3.9
A/G Ratio 0.7 − 1.7
Overview:
Detect and identify monoclonal immunoglobulin gammopathies. Diagnose myeloma, macroglobulinemia of Waldenström. Immunofixation is used to evaluate monoclonal gammopathy (M protein) found on serum protein electrophoresis; evaluate amyloidosis. Has application to the evaluation of lymphoproliferative disease (malignant lymphoma and others) and collagen disease in general. Of use in the diagnosis and characterization of immune deficient and dysgammaglobulinemic states. The NCCN Clinical Practice Guidelines in Oncology (NCCN Guidelines™) recommend the use of serum free light chain assays for use in the initial diagnostic workup of multiple myeloma and related disorders. "Use of free light chain (FLC) assay along with SPEP and SIFE yields high sensitivity while screening for MM and related plasma cell disorders. Therefore, this assay is now included as a part of the initial diagnostic workup in the NCCN Multiple Myeloma Guidelines."1
1. NCCN Clinical Practice Guidelines in Oncology (NCCN Guidelines™) for Multiple Myeloma, Version 1.2011. Fort Washington, Pa: NCCN; 2011.
Patient Preparation:
Patient should be fasting for eight hours to avoid lipemic sample interference.
Collection Instructions:
Red-top tube or gel barrier tube.
Sample should be allowed to clot and the serum separated as soon as possible to prevent hemolysis.
Refrigerate at 2°C to 8°C.
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