Turnaround Time: 7 Days
CPT Code:

82670, 84244, 84402

Test Type: Kit Based, Salivary


The Rhythm hormone test is a comprehensive assessment of estradiol, progesterone, and testosterone spanning a full 28 days. Hormone imbalances revealed in this hormone test profile can help illuminate root causes of disorders such as premenstrual syndrome (PMS), infertility, and menstrual irregularities. This hormonal test analyzes 11 saliva samples of a 28–day period.

Hormonal balance is the key. Research has long shown that fluctuating levels of estradiol, progesterone, and testosterone play a major role in a woman's overall health affecting:

  • Menstrual cycle
  • Mood swings
  • Sleep patterns
  • Appetite level
  • Sex drive


Chronic hormonal imbalances are implicated in disorders such as:

  • PMS
  • Anovulation
  • Infertility
  • Amenorrhea
  • Endometriosis
  • Polycystic Ovary Disease
  • Osteoporosis


Saliva hormone testing provides a thorough analysis of estradiol and progesterone over a full 28 day cycle. Testosterone is measured once from the 28th day specimen. By utilizing 11 saliva samples for analysis, the relationship and balance of these essential hormones are analyzed more precisely through time. The levels of estradiol and progesterone, as well as the ratio between the two, are clearly graphed for easy reference and patient education.

Unlike serum measurements that typically reflect both bound and unbound fractions of hormones, salivary samples represent only the free (unbound) bioavailable fraction of hormone. Factors that affect binding globulin such as obesity and thyroid function do not influence test results with false high or low test levels.

The Rhythm profile is a hormonal test indicated for both premenopausal and perimenopausal women not currently supplementing with hormones. An analysis of estrogen, progesterone and testosterone can reveal ovulatory function and trends in hormone production. Hormonal imbalances such as unopposed estrogen, high follicular progesterone, anovulation and luteal phase defects are easily identified. This hormone test is especially useful in treatment of patients with chronic gynecologic disorders.

Collection Details:

Patient Preparation:

Important things to know and consider:
• Transdermal (cream) and sublingual bio-identical hormones may produce
artificially high levels in the saliva that do not correlate with blood levels. This increase from cream hormones may last for weeks to months after
discontinuing use. If you are taking these substances – or have taken them within the last 12 months – please consult with your healthcare practitioner
before taking this test.
• The following drugs, herbs and dietary supplements may influence levels of hormones reported in this test: melatonin (at doses greater than 5 mg),
ketoconazole, cimetidine (Tagamet), anastrozole (Arimidex), letrozole (Femara),
exemestane (Aromasin), digoxin, antiepileptics, Chrysin, Apigenin, and Tribulus terrestris. Let your physician know about these and any other medications, herbs, and supplements that you have used in the past 3 months. Do not change use of supplements or medications unless instructed to do so by your healthcare provider.
Schedule and prepare for your saliva collection:
• Your collection schedule is based on the first day of your menstrual cycle (the day menstrual blood flow begins). If you are not experiencing periods, you may start on any day. You may find it helpful to use a calendar to remind you of
collection dates.
•It is important that you collect saliva during the specified time frame.
•If you have difficulty producing enough saliva for the tube, press the tip of your tongue to the roof of your mouth against your teeth. Yawning can also
generate saliva.
• One hour prior to collection do not eat, brush or floss your teeth, use mouthwash, chew gum or use any tobacco products. You may drink ONLY water during this time-

Collection Instructions:

Collecting Saliva Specimens: Not following these instructions may affect your test results.

1. Write your full name, time, and date of collection on label #1 using a ballpoint pen or pencil only. Attach the label to a collection tube.

2. When ready to collect the saliva, rinse your mouth with drinking water. Spit out the water completely, and do not cough or clear your throat into the collection tube.

3. Fill the collection tube until liquid saliva level reaches the 3-ml mark. To reduce a
foamy saliva collection, tap the tube on a hard surface. Insufficient saliva samples cannot be processed.

4. Replace the cap tightly to avoid leakage. Place the filled tube into the biohazard bag and
freeze immediately. Samples must be frozen a minimum of 2 hours prior to shipping. Keep
sample frozen until ready to ship. Repeat steps 1-4 for each collection, using the labels numbered 2 and 3, etc., in order.