Rotavirus Antigen, Direct Detection Immunoassay

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Turnaround Time: 1 - 4 days
CPT Code:


Test Type: 2 mL liquid stool or 1 g semiformed stool
Reference Range:



Detect rotavirus in stools of patients suspected of having viral gastroenteritis.

Quality of specimens cannot be evaluated, and specimens are collected randomly.

Rotavirus is an extremely common cause of pediatric gastroenteritis. The illness is most common in winter, is highly contagious, involves five to eight days of diarrhea, and is rarely fatal.1 Patients should also be evaluated for possible bacterial gastroenteritis. Outbreaks are seen among children in daycare centers.

1. Christensen ML, Howard C. Viruses causing gastroenteritis. In: Balows A, Hausler WJ, et al, eds. Manual of Clinical Microbiology. 5th ed. Washington, DC: ASM Press;1991:950-958.
Christensen ML. Human viral gastroenteritis. Clin Microbiol Rev. 1989 Jan; 2(1):51-89. PubMed 2644024

Gray LD. Novel viruses associated with gastroenteritis. Clin Microbiol Newslet. 1991; 13(18):137-144.

Collection Details:

Collection Instructions:

Empty stool transport vial or screw-cap container, sterile no additives.

Collect as soon as possible after onset of disease, preferably three to five days after onset. Collection from diapered children can be facilitated by using a disposable diaper “inside out” or by using a “Saran wrap” diaper to capture the next stool specimen.

Refrigerate at 2°C to 8°C immediately after collection.