TRIAD Profile (Bloodspot)

Collection Details:

Patient Preparation:

Patient Preparation:

Blood Spot, Non-Fasting - 

• It is best to ship your specimen within 48 hours of collection. Please refer to the enclosed shipping instructions before you collect to determine what days you can ship your specimen.
• It is not necessary for the patient to fast
• It is not necessary to discontinue nutritional supplements prior to this test. Abnormalities that may be found will reveal special needs that have not been met by recent dietary and supplemental intake.
• The use of immunosuppressive drugs, such as cortisone, can give false negative results. Discontinue the use of such drugs for 60 days before testing to allow antibody reactions to be seen.

Urine - 

• It is best to ship your specimen within 48 hours of collection. Please refer to the enclosed shipping instructions before you collect to determine what days you can ship your specimen.
• It is not necessary to discontinue nutritional supplements prior to this specimen collection. Abnormalities that may be found will reveal special needs that have not been met by recent dietary and supplemental intake.
• Decrease fluid intake to avoid excessive dilution of the urine - For adults, restrict intake to three 8 oz. glasses or less for 24 hours - Make sure that no more than 8 oz. of this is consumed after 8:00 PM the evening prior to urine collection
• Do not collect urine during menstruation
• Vial contains preservative - Do Not Rinse

Blood Spot, Fasting - 

• It is best to ship your specimen within 48 hours of collection. Please refer to the enclosed shipping instructions before you collect to determine what days you can ship your specimen.
• Patient must be fasting for 8 hours. Patient may have water.
• It is not necessary to discontinue nutritional supplements prior to this test. Abnormalities that may be found will reveal special needs that have not been met by recent dietary and supplemental intake.

Collection Instructions:

Urine Collection

1. Write patient’s first and last name, date of birth, and date of collection on
the Test Requisition Form (located in the pouch on top of the Specimen Collection Kit Box), as well as on clear cap plastic vial, using a permanent marker.

2. Empty bladder before going to bed at night. Do not collect this urine.

3. Collect urine (if any) during the night and first morning urine into a clean container.

4. Pipette urine, using a fresh disposable pipette, into the clear cap plastic vial to the 12 ml mark (DO NOT OVERFILL). Screw the cap on tightly.

5. Dispose of remaining urine.

6. Freeze the clear cap plastic vial and ice pack.

7. Continue to Step 3, the fasting blood spot specimen collection.

Bloodspot finger stick collection card:

1. Write patient’s first and last name, date of birth, gender and date of collection on the Test Requisition Form (located in the pouch on top of the Specimen Collection Kit Box), as well as where indicated on the absorbent collection card with the four circles, using a permanent marker.
• IMPORTANT: To ensure accurate test results you must provide the requested
information.

2. Complete the Test Requisition Form. Fill in “Date Specimen
Collected” on the Test Requisition Form under section #4. Fold and place the form inside the postage paid envelope.

3. Remove the clear cover from the lancet. One end of the lancet
has a small hole in the center; this will be the end that you push against your finger to engage the needle.

4. Warm your hands under warm water for a few minutes. Dry hands, then select your middle finger. Gently massage the entire length of the finger to increase the temperature and improve circulation.

5. Clean the tip of your finger with the alcohol pad.

6. Hang your arm down and gently shake your fingers a few seconds to increase blood pools in the finger.

7. Hold your hand lower than heart level; again gently massage the lower portion of the finger. Firmly grasp the lower portion of the finger for a few seconds to restrict return circulation.

8. Choose a site on the middle fingertip. Push the red tip end of the lancet firmly against your fingertip to depress the tip and release the needle. The needle will immediately retract safely after depression.

9. Dispose of the lancet in a suitable container (out of the reach of children and pets).

10. Using your thumb, gently massage entire length of the pricked finger to form repeated blood drops. As each drop forms, touch the tip of the drop to a circle on the absorbent collection card. Do not smear.
DO NOT TOUCH FINGERTIP TO THE CARD.

11. Repeat until blood has soaked to the border of the circle on the absorbent
collection card. Continue this procedure for all Four circles. Check the back of the card. Compare all blood saturated circles to the illustration shown as “YES” (see insert). Both the front and back of the card should look the same as the “YES” illustration.

12. If you are unable to get sufficient blood from the first collection, Return to step
3 using a different finger. To increase blood pools in the finger, the yellow rubber
band may be used. After you warm your hands under water for a few minutes, Wrap the yellow rubber band twice around your forearm - about where you would wear a watch, then proceed to step 5. When finished, remove the yellow rubber band.
13. If necessary, use the absorbent pad to dry the site on your finger after your collection. Use the enclosed adhesive bandage as needed.
14. Allow absorbent collection card to air dry overnight, approximately 24 hours,
before placing in the resealable plastic bag with desiccant/moisture absorbent pack for shipping. If the card is not completely dry, your sample may be unusable.