Coombs', Direct (DAT)

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Turnaround Time: 1 - 2 days
CPT Code:


Test Type: 4 mL Whole blood
Reference Range:



Detect in vivo coating of IgG and/or complement components on RBCs.

The presence of potent cold autoagglutinins may interfere with test interpretation. Testing performed using clotted blood specimens may yield false-positive results with anticomplement.

Positive direct antiglobulin tests are associated with a variety of conditions which include hemolytic transfusion reaction, autoimmune hemolytic anemia, hemolytic disease of the fetus/newborn, and may be secondary to certain pathological conditions or drug therapy. Note: The presence of a positive direct antiglobulin test does not necessarily correlate with a pathological condition.

Fung MK, Grossman JB, Hillyer CD, Westoff CM, eds. AABB Technical Manual. 18th ed. Bethesda, Md: American Association of Blood Banks;2014.

Collection Details:

Collection Instructions:

Lavender-top (EDTA) tube or yellow-top (ACD) tube.

Specimens should be tested as soon as possible or within 72 hours of collection. Samples that cannot be tested immediately should be stored at 2°C to 8°C. Do not freeze.